Identification and properties of a sterol-binding polysaccharide isolated from Saccharomyces Cerevesiae

A sterol-solubilizing polysaccharide isolated from yeast has been described (Adams, B. G. and Parks L. W. (1967) Biochem. Biophys. Res. Commun. 28,490–494) which greatly simplifies the addition of sterolsto aqueous media. The polysaccharide has now been identified by gas-liquid chromatography and carbazole reactions as yeast cell wall mannan. The cell wall mannan has been purified and binding constants for several sterols have been determined. Binding of sterols is a biphasic function of mannan concentration and is independent of pH over a range of 5 to 8.     

Dq−, multiple Mißbildungen und Retinoblastom

Summary A child has been described who developed a retinoblastoma in the left and probably also in the right eye at the age of two years. Mitosis from peripheral blood cells and fibroblasts showed a deletion of the long arm of a D-group chromosome which could be identified by autoradiography as D₁. The child died at the age of 2 1/2 years. An autopsy could not performed.